Survey of cyanomyovirus abundance in Shantou coastal waters by g20     

李传标  丁俊  周立镇  张昭  李升康  刘文华  温小波 《中国海洋湖沼学报》2015,33(3):604-615

To understand the genetic diversity and population changes in cyanophages in the coastal waters of Shantou,northeast South China Sea,we used the capsid assembly protein gene g 20 as a marker of the abundance and phylogeny of natural cyanomyovirus communities. The abundance of total viruses,heterotrophic bacteria,and picophytoplankton in the coastal waters was monitored with flow cytometry. Hydrological parameters(NO ˉ 3,NO ˉ 2,NH 3,soluble reactive phosphorus,total dissolved nitrogen,total dissolved phosphorus,dissolved oxygen,chemical oxygen demand,temperature,salinity,and chlorophyll a concentration) and microbial abundance(total viruses,total bacteria,P rochlorococcus,Synechococcus,and eukaryotes) were measured in the upper and lower layers at four sampling sites in the research area. In the direct viral counts,cyanomyoviruses accounted for 1.92% to 10% of the total viral community. A phylogenetic analysis showed that the g 20 sequences in the Shantou coastal waters were very diverse,distributed in eight distinct operational taxonomic units,including the newly formed Cluster W. The g 20 gene copies inferred from real time PCR assay indicated that cyanomyoviruses were correlated significantly with the heterotrophic bacteria numbers and the nitrate and chlorophyll a concentrations. These results suggest that cyanomyoviruses are ubiquitous and are an abundant component of the virioplankton in Shantou coastal waters.  相似文献   

Molecular cloning of the heat shock protein 20 gene from Paphia textile and its expression in response to heat shock     

李佳凯  武祥伟  谭茎  赵瑞祥  邓凌韦  刘贤德 《中国海洋湖沼学报》2015,33(4):919-927

P. textile is an important aquaculture species in China and is mainly distributed in Fujian, Guangdong, and Guangxi Provinces. In this study, an HSP20 c DNA designated Pt HSP20 was cloned from P. textile. The full-length c DNA of Pt HSP20 is 1 090 bp long and contains a 5′ untranslated region(UTR) of 93 bp, a 3′ UTR of 475 bp, and an open reading frame(ORF) of 522 bp. The Pt HSP20 c DNA encodes 173 amino acid residues and has a molecular mass of 20.22 k Da and an isoelectric point of 6.2. Its predicted amino acid sequence shows that Pt HSP20 contains a typical α-crystallin domain(residues 77–171) and three polyadenylation signal-sequences at the C-terminus. According to an amino acid sequence alignment, Pt HSP20 shows moderate homology to other mollusk s HSPs. Pt HSP20 m RNA was present in all of the test tissues including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, with the highest concentration found in the gonad. Under the stress of high temperature, the expression of Pt HSP20 m RNA was down-regulated in all of the tissues except the adductor muscle and gonad.  相似文献   

Experimental research on mechanical characteristics and strength criterion of rock of triaxial unloading tests under different stress pathsEI北大核心CSCD     

Zhao Guo-yanDai BingDong Long-junYang Chen 《岩土力学》2015,(11):3121-+

By carrying out triaxial unloading test under different stress paths on rock, the complete stress-strain curves, deformation characteristics and its strength criterion are studied. The test results show that the relation between lateral strain and confining pressure firstly is linear, and then is nonlinear in the total stage of unloading confining pressure. And its growth rate is about 3 to 5 times the growth rate of the axial strain. It shows obvious lateral dilatancy, and the degree of dilatancy is related to the unloading path. According to the area that is enclosed by confining pressure and volumetric strain curve, it can be seen that the greater the confining pressure is before unloading, the more the energy releases. Deformation modulus decreases gradually with the unloading confining pressure, and increases with the initial confining pressure; the relation between deformation modulus and confining pressure shows negative exponential distribution overall. At the same unloading paths, the reduction of the deformation modulus increases with the increase of initial confining pressure. The Poisson's ratio rises up continually, and the variation law with the confining pressure can be well described by nonlinear. Failure characteristics of rock are mainly shear failure. The strength characteristics of rock can be well characterized by using the Mohr strength criterion of power function. These conclusions provide reliable theoretical reference and guiding significance for the deep mining of the underground metal mine.  相似文献   

The mechanics of continental extension in Qiongdongnan Basin,northern South China Sea     

Zhongxian Zhao  Zhen Sun  Zhenfeng Wang  Zhipeng Sun 《Marine Geophysical Researches》2015,36(2-3):197-210

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南海北部琼东南盆地陵水段峡谷沉积建造及勘探意义   总被引：7，自引：6，他引：1  

张道军  王亚辉  赵鹏肖  苏榆丰  左倩媚 《海洋学报》2015,37(2):25-35

琼东南盆地中央峡谷为一大型轴向峡谷体系,具有明显的"分段性"特征,不同区段沉积充填和内部结构均存在明显的差异,其中陵水段位于峡谷西段,具有非常好的储层物性特征,是深水区峡谷勘探的重点区域。为了进一步精细刻画陵水段峡谷内部水道复合体的沉积充填及沉积微相展布特征,本文借助新增加的三维地震资料和新钻井资料,通过峡谷三级层序界面的识别,将峡谷充填划分为3个三级层序SQ1、SQ2和SQ3,并通过古生物有孔虫化石带、钙质超微化石带及井-震结合确定了峡谷底界为中中新世晚期S40界面,陵水段峡谷自西向东依次识别出6种主要的内部充填结构,且将SQ3层序细分为5个次级层序(SSQ1~SSQ5);结合属性特征,精细刻画了陵水段峡谷内沉积微相平面展布及空间演化特征,并指出点砂坝在SSQ1层序最发育,且规模较大,横向上连片分布,将是下步勘探的有利储集体。  相似文献   

历次核试验进入海洋的~(137)Cs对中国近海影响的模拟研究   总被引：1，自引：0，他引：1  

赵昌  乔方利  王关锁  舒启  夏长水 《海洋学报》2015,37(3):15-24

1945年以来,世界各主要核国家进行了数千次核试验,这些核试验产生的绝大部分放射性物质通过多种途径进入海洋,对海洋环境造成放射性污染。本文建立了一个准全球海洋的放射性物质输运和扩散数值模式,通过数值模拟手段评估了历史核试验释放的放射性物质137 Cs对中国近海海洋环境的影响。本文借助前人工作评估了核试验释放137Cs进入海洋的途径和总量;通过比较模拟结果与观测资料,表明本文建立的放射性物质模式能够较好地模拟出137Cs在中国近海及其邻近海域的分布情况和随时间演变特征;模拟结果表明中国近海里的137Cs浓度在20世纪50年代中期达到最大,其中吕宋海峡海域137Cs浓度最高,达80.99Bq/m3;进一步分析了2011年3月份日本福岛核事故前中国近海137Cs浓度分布状况,2011年整个中国近海137Cs浓度介于1.0~1.6Bq/m3间,且其浓度垂向分布较均匀,相对封闭的南海浓度略高于其他海域。  相似文献   

南极半岛东北海域表层沉积有机质来源及其沉积环境     

韩喜彬  赵军  初凤友  潘建明  唐灵刚  许冬  边叶萍  葛倩 《海洋学报》2015,37(8):26-38

采用气相色谱-质谱技术对南极半岛东北海域海底表层沉积物的总有机碳、有机质碳同位素(δ13 Corg)和生物标志化合物等进行了测试分析。研究区表层沉积物总有机碳TOC平均值高于现代深海沉积物中的平均含量。δ13 Corg的变化说明该海域有机碳来源呈海洋水生生物来源和陆源混合的特征。正构烷烃的峰型分布、主峰碳、饱和烃轻重比C-21/C+22和(C21+C22)/(C28+C29)、甾烷组合和藿烷组合证实研究区西部表层沉积物有机质来源以陆源高等植物为主,其陆源可能来自于附近的南极半岛和南舍得兰群岛;研究区东部表层沉积物有机质来源偏以海源为主,且以低等浮游生物、藻类及细菌生物等海源输入为主。碳优势指数(Carbon preference index,CPI)、奇偶优势指数(Odd-even Predominance,OEP)和甾烷C29ααα20S/(20S+20R)比值显示研究区D1-7站位和D5-9沉积物有机质演化程度较高,D5-2和D2-4站位的有机质演化程度低,其他站位介于中间状态。饱和烃中姥鲛烷、植烷及其比值(Pr/Ph)等组合显示研究区西部以氧化-弱还原的沉积环境为主,其可能是受高温低盐别林斯高晋海水流和附近火山喷发的影响所致;研究区东部以还原—强还原沉积环境为主,可能是受低温高盐的威德尔底层水(WSBW)和威德尔海深层水(WSDW)影响所致。  相似文献   

北极中央区海冰密集度与云量相关性分析   总被引：2，自引：0，他引：2  

纪旭鹏  赵进平 《海洋学报》2015,37(11):92-104

本文使用海冰密集度以及低云、中云、高云的日平均数据,借助滑动相关分析方法,研究了北极中央区海冰密集度与云量之间的相关性,分析了海冰与云的相互作用机制。研究表明,在春季海冰融化季节(4、5月)、秋季海冰冻结季节(10、11月),低云与海冰密集度之间表现为较好的负相关,表明在这段时间内冰区海面蒸发强烈,对低云的形成有重要贡献。在10月和11月,中云与海冰密集度也有很好的负相关,表明秋季低云可以通过抬升形成中云。高云与海冰密集度之间并没有明显的相关性,可能原因:一方面海冰的空间分布对高云无影响,另一方面,高云主要影响到达的短波辐射,从而影响海冰的融化和冻结速度,与海冰厚度有直接显著的关系,而与海冰密集度的关系不明显。此外,在海冰密集度与低云存在较好负相关的情况下会出现某些年份相关性不好的情况,我们的研究发现这是北极中央区与周边海区发生了海冰交换或云交换的结果。  相似文献   

Temporal variability of the current in the northeastern South China Sea revealed by 2.5-year-long moored observations   总被引：1，自引：0，他引：1  

Li Cheng  Zhiwei Zhang  Wei Zhao  Jiwei Tian 《Journal of Oceanography》2015,71(4):361-372

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A long-term volume transport time series estimated by combining in situ observation and satellite altimeter data in the northern South China Sea   总被引：1，自引：0，他引：1  

Xiao-Hua Zhu  Ruixiang Zhao  Xinyu Guo  Yu Long  Yun-Long Ma  Xiaopeng Fan 《Journal of Oceanography》2015,71(6):663-673

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